Academic paper homework help question microbiology lab questions question 1 you are reading culture plates today and identifying pathogens that grow from patient samples. Essay's paper body one side of the bhia medium was inoculated with the gram positive cocci and the other side with the gram negative unknown bacteria, completing this section of lab by placing the labeled medium into the 350c incubator for 48 hours. Gram staining from wikipedia, the free encyclopedia a gram stain of mixed staphylococcus aureus (staphylococcus aureus atcc 25923, gram-positive cocci, in purple) andescherichia coli (escherichia coli atcc 11775, gram-negative bacilli, in red), the most common gram stain reference bacteria gram staining (or gram's method) is a method of differentiating bacterial species into two large groups.
Read this essay on gram staining come browse our large digital warehouse of free sample essays get the knowledge you need in order to pass your classes and more. Gram staining: it differentiates between two large groups of microorganisms due to their cell wall differences: pink-staining gram- cells, and purple-staining gram+ cells this preview has intentionally blurred sections. Gram staining lab introduction gram staining is a very important technique used in biology labs all over the world it is a technique used to differentiate types of bacteria using certain physical and chemical characteristics of their cell walls.
Identification of a unknown bacterium essay sample the gram stain was accomplished by preparing a smear and applying crystal violet to it, and allowing it react. Bacterial classification by gram staining pages 4 words 764 view full essay more essays like this: sign up to view the complete essay show me the full essay. We will write a custom essay sample on gram positive bacteria specifically gram stain test can be used to easily distinguish between a gram positive and gram. In the gram stain, what dye is primary stain, which is the counter stain describe the molecular basis behind gram staining only ma/ms and phd essay writers. Gram stain this separation is based on differences in what physical trait the structure of the cell walls what is the iodine used for in this staining technique.
The main objective of this lab was to identify different bacteria by simple, negative, and gram staining to view each bacteria cell, the bacteria was transferred aseptically to a slide, and they were then viewed by using oil immersion, by a light microscope from this lab, it was determined that e. The gram stain is a useful stain for identifying and classifying bacteria the gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. The purpose of this experiment was to learn how to properly perform a gram stain and to also understand how gram staining can help identify either gram-positive or gram-negative organisms in the laboratory.
The gram stain differentiates bacteria into two fundamental varieties of cells bacteria that retain the initial crystal violet stain (purple) are said to be gram-positive, whereas those that are decolorized and stain red with carbol fuchsin (or safranin) are said to be gram-negative. A narrative type of essay refers to a written we are online 24/7 introduction gram staining is the most essential and universally used staining technique in bacteriology laboratory. Read this essay on gram stain come browse our large digital warehouse of free sample essays get the knowledge you need in order to pass your classes and more only at termpaperwarehousecom.
The thick peptidoglycan layer of the cell wall of gram positive bacteria retain the crystal violet dye and stain purple even after decolourisation but for gram negative bacteria, it is decolourised by the decolourising agent. Experiment 2 simple stain and the gram stain in most microbiological staining procedures, the bacteria are first fixed to the slide by the heat fixed smear (figure 1)in this procedure living, potentially pathogenic bacteria are. The bv score1 is calculated based upon the gram stain results in the cell wall and is an important taxonomic feature, gram staining is therefore used.